Identification of chalcone isomerase family genes and roles of <i>DnCHI1</i> in flavonoid metabolism in <i>Dendrobium nobile</i>.

ABSTRACT: Dendrobium nobile is a prized orchid species with both medicinal and ornamental values, known for accumulating flavonoids that contribute to its bioactivity and flower pigmentation. Chalcone isomerase (CHI), a key enzyme in the flavonoid biosynthetic pathway, catalyzes the conversion of chalcone to naringenin, thereby promoting the production of anthocyanins and other flavonoids. In this study, we present the first systematic analysis of the CHI gene family in D. nobile, classifying them into two subfamilies. Expression profiling revealed that DnCHI1, a type IV CHI, is highly expressed in petals, suggesting a potential role in anthocyanin metabolism. Heterologous transient overexpression assays showed that DnMYB90 significantly downregulated

SUBMITTER: Chang LL 

PROVIDER: S-EPMC12660066 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature