Inducible gene expression in Lactobacillus reuteri LTH5531 during type II sourdough fermentation.

ABSTRACT: Lactobacillus reuteri LTH5531 is a dominant member of the microbiota of type II sourdough fermentations. To investigate the genetic background of the ecological performance of LTH5531, in vivo expression technology was used to identify promoters that show elevated levels of expression during growth of this organism in a type II sourdough fermentation. Thirty-eight sourdough-induced fusions were detected, and 29 genes could be identified on the basis of the available sequence information. Four genes encoded stress-related functions (e.g., acid and general stress response), reflecting the harsh conditions prevailing during sourdough fermentation. Further, eight genes were involved in acquisition and synthesis of amino acids and nucleotides, indicating their limited availability in sourdough.

SUBMITTER: Dal Bello F 

PROVIDER: S-EPMC1266011 | biostudies-literature | 2005 Oct

REPOSITORIES: biostudies-literature