Spatial transcriptomics reveals TGF-β-driven endothelial-mesenchymal transition in vascular remodeling of moyamoya disease.

ABSTRACT: Current understanding of molecular mechanisms driving moyamoya disease (MMD) remains limited. This study aimed to elucidate the role of TGF-β signaling in MMD vascular remodeling via endothelial-to-mesenchymal transition (EndMT). Spatial transcriptomics (10x Visium) was performed on 11 MMD specimens, while hematoxylin-eosin (HE) staining, immunohistochemistry (lHC), and immunofluorescence were conducted on a total of 51 MD and 11 control vascular specimens. Pseudotemporal trajectory analysis and cell-cell communication modeling were employed to map EndMT dynamics. MMD vessels exhibited intimal hyperplasia (STA: N1/N = 0.38 ± 0.12 vs 0.25 ± 0.12, p < 0.01) and medial atrophy. Spatial transcriptomics identified dual-positive SMC-Endo clusters (13.6%) within hyperplastic

SUBMITTER: Xiao J 

PROVIDER: S-EPMC12660125 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature