<i>Ex vivo</i> perfusion model of mouse liver and its application to analyze the effects of OCT1 deficiency.

ABSTRACT:

Introduction

The liver plays a critical role in drug pharmacokinetics. In in vivo experiments, it is difficult to isolate the liver's contribution to drug systemic concentrations from that of the intestine and kidneys. Rat liver perfusion is well-established for studying liver-specific effects. However, rats are not easily genetically manipulated, complicating analyses of individual drug transporters and metabolizing enzymes. This study aimed to establish an ex vivo liver perfusion model in mice and to apply it to analyze the effects of mOct1 on drug metabolism.

Methods

After euthanizing, the liver of 6- to 28-week-old mice was perfused via an indwelling venous catheter in the portal vein as entry and into the caudal vena cava toward the heart as exit. Perfusio

SUBMITTER: Ronnpagel V 

PROVIDER: S-EPMC12660287 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature