Commensal Peptoniphilus harei induce activation of monocytes via TLR2/CD14 signalling in whole blood.

ABSTRACT: Myeloid cells, such as monocytes, are central in the immune response to infections. However, limited knowledge exists regarding the inflammatory response to Gram-positive anaerobic cocci (GPAC).To explore the ability of GPACs to induce monocyte activation, we compared two GPAC species, Parvimonas micra and Peptoniphilus harei, with E. coli, a major cause of bloodstream infections. We stimulated whole blood with heat-killed bacteria or conditioned medium (CM) and analysed monocytes for signs of activation by flow cytometry. The mechanisms were explored using anti-CD14- or anti-TLR2 antibodies. Finally, to investigate potential pathogen-associated molecular patterns in CM, we performed mass spectrometry (MS).Our findings revealed that P. harei significantly induced activation of monocytes, a

SUBMITTER: Schmidt T 

PROVIDER: S-EPMC12660362 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature