Nymphal RNAi: systemic RNAi mediated gene knockdown in juvenile grasshopper.

ABSTRACT:

Background

Grasshopper serves as important model system in neuroscience, development and evolution. Representatives of this primitive insect group are also highly relevant targets of pest control efforts. Unfortunately, the lack of genetics or gene specific molecular manipulation imposes major limitations to the study of grasshopper biology.

Results

We investigated whether juvenile instars of the grasshopper species Schistocerca americana are conducive to gene silencing via the systemic RNAi pathway. Injection of dsRNA corresponding to the eye colour gene vermilion into first instar nymphs triggered suppression of ommochrome formation in the eye lasting through two instars equivalent to 10-14 days in absolute time. QRT-PCR analysis revealed a two fold decrease of target tran

SUBMITTER: Dong Y 

PROVIDER: S-EPMC1266053 | biostudies-literature | 2005 Oct

REPOSITORIES: biostudies-literature