Cathepsin B as a potential serum biomarker for early diagnosis and progression of diabetic foot ulcer complicated with peripheral vascular disease.

ABSTRACT: The existing diagnostic methods of diabetic foot ulcer (DFU) complicated with peripheral vascular disease (PVD) lack sufficient potential for early identification, which leads to slow wound healing, amputation and even death. Thus, this study aimed to explore the potential serum biomarkers of DFU complicated with PVD. A target gene of DFU complicated with PVD was identified using single-cell transcriptome analysis. The immunohistochemistry, ELISA, clinical correlation analysis, tubulogenesis assay, CCK8 assay, and scratch assay were used to verify the correlation between this target gene and DFU complicated with PVD. The ELISA experiment was used to detect the target gene in serum. In this study, the result of PPI in single-cell transcriptomes showed that cathepsin B (CTSB) was enriched in

SUBMITTER: Jin Y 

PROVIDER: S-EPMC12660663 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature