Wound healing improvement by a multicomponent wound dressing of keratinocyte-imprinted polydimethylsiloxane substrate in a rabbit model.

ABSTRACT: Wound healing is a major clinical challenge and regenerative medicine and tissue engineering suggest new ways to overcome such problems. The cell imprinting method can provide cues that regulate cell function or modulate stem cell differentiation into different lineages. In this study, the rabbit keratinocyte-imprinted polydimethylsiloxane (PDMS) substrate (KiPDMS) was made and associated with adipose-derived stem cells (ADSCs), keratinocytes, and collagen scaffold, provided various treatments. After evaluating ADSCs' differentiation into keratinocytes by qPCR and immunocytochemistry techniques, different treatments were implanted in a rabbit ear, histopathological analysis was performed on measuring the epidermal thickness and neovascularization. The histopathological analysis revealed th

SUBMITTER: Alipour S 

PROVIDER: S-EPMC12660737 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature