A high-precision nanoliter droplet dispensing system based on optoelectrowetting with tunable droplet volume.

ABSTRACT: The capacity to generate high-precision droplets within Lab-on-a-Chip (LOC) devices is essential for numerous biochemical applications, such as DNA sequencing and drug delivery. In this study, we introduce an optoelectrowetting (OEW)-based droplet manipulation system that utilizes a novel droplet dispensing strategy, enabling precise nanoliter droplet dispensing with tunable droplet volume. The system comprises an OEW microchip, a liquid crystal display (LCD) projector connected to a laptop for generating customized light patterns, and a microscope equipped with a charge-coupled device (CCD) camera mounted above the OEW microchip for real-time observation. Simulations and experiments were conducted to investigate the optimal conditions for high-precision droplet dispensing. The system demo

SUBMITTER: Huang K 

PROVIDER: S-EPMC12660984 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature