Optimization of Aspergillus Niger fermentation for enzyme production and enzymatic hydrolysis of wheat bran for total ferulic acid via RSM.

ABSTRACT: Phenolic acids in wheat bran, particularly ferulic acid (FA), exhibit antimicrobial properties, but most FA is bound to cell wall polysaccharides via ester/ether linkages, severely limiting its bioavailability and extraction. Distinct from prior studies relying on commercial enzymes or single-component substrates, this study enhanced FA release through Aspergillus niger (A. niger) fermentation (no exogenous enzymes), utilizing a wheat bran-vinegar residue (9:1, w/w) substrate. Fermentation and destarched wheat bran (DSWB) enzymatic hydrolysis were optimized via Box-Behnken design and response surface methodology (BBD-RSM), yielding high enzyme activities: feruloyl esterase (16.63 ± 0.32 U/g), xylanase (3098.21 ± 47.27 U/g), and cellulase (21.12 ± 0.14 U/g). Hydrolysis released 5.41 ± 0.03 

SUBMITTER: Chen L 

PROVIDER: S-EPMC12661051 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature