Functional genome analysis reveals that serine carboxypeptidase <i>Bd-SCP10</i> mediates vegetative growth, pathogenicity, and stress tolerance in <i>Botryosphaeria dothidea</i>.

ABSTRACT:

Introduction

Botryosphaeria dothidea (B. dothidea) is a catastrophic fungal pathogen that threatens fruit production worldwide. Secreted peptidases like serine carboxypeptidases (SCPs) are well known to be involved in fungal virulence, but their role in B. dothidea is unknown.

Methodology

Here, we identified and functionally characterized Bd-SCP10, a homolog of SCPs found in B. dothidea, which is a member of the S10 family, using a split marker strategy for gene knockout and complementation.

Results

Mutants exhibited substantial phenotypic changes, including reduced radial growth and compromised biomass production, as well as altered pathogenicity and stress tolerance in response to multiple stress conditions. In

SUBMITTER: Umer M 

PROVIDER: S-EPMC12661404 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature