Comparative evaluation of analytical methods for CSF proteomics.

ABSTRACT: Cerebrospinal fluid (CSF) provides a unique window into brain pathology, yet challenges in unbiased mass-spectrometric (MS) discovery persist due to sample complexity and the need for optimized analytical workflows. Multiple laboratory workflows have been developed for CSF proteomics, each with distinct advantages for specific applications. To interrogate which laboratory workflow is most suitable for this biological matrix, we benchmarked five orthogonal sample-preparation strategies- MStern, Proteograph™ nanoparticle enrichment (Seer), N-glycopeptide capture (N-Gp), and two extracellular-vesicle (EV) fractions isolated by differential ultracentrifugation (P20- and P150-EV)- in CSF from 19 patients with central nervous system lymphoma. The protocols span a practical spectrum of input volu

SUBMITTER: Aastha A 

PROVIDER: S-EPMC12661759 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature