TET1-Mediated DNA hydroxymethylation of KLF6 promotes LPS-induced pulmonary fibrosis by activating the S1PR3/RhoA/ROCK signaling pathway.

ABSTRACT:

Background

Ten-Eleven Translocation methylcytosine dioxygenase 1 (TET1) plays crucial roles in organ injury and fibrosis, but its role and regulatory mechanism in pulmonary fibrosis remain to be elucidated.

Methods

Gene and protein expression levels were assessed using RT-qPCR, Western blot and immunohistochemistry. CCK-8 and TUNEL were used to detect cell viability and apoptosis, respectively. The DNA methylation of Kruppel-like factor 6 (KLF6) was detected by MSP. 5-hydroxymethylcytosine (5hmC) level of KLF6 was detected by the corresponding kit. ChIP assay or dual luciferase reporter gene assay was applied to detect the binding relationship between TET1 and KLF6 promoter/KLF6 and Sphingosine-1-phosphate receptor 3 (S1PR3) promoter. Histological examination, including H&E

SUBMITTER: Chen J 

PROVIDER: S-EPMC12661897 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature