Properties of Novel Components of Polysaccharides Isolated From <i>Lycium barbarum</i> Fruits.

ABSTRACT: This study has established structure-function relationships of Lycium barbarum polysaccharides (LBPs) relevant to antioxidant and tyrosinase-inhibitory functions in food systems by integrating optimized extraction, stepwise purification, comprehensive structural analysis, and functional verification, offering a novel multimethod approach to explore structure-based mechanisms of bioactivity. Firstly, hot-water extraction optimized at 30 mL/g, 90°C, and 5 h yielded 7.44% ± 0.17% crude LBPs. Secondly, ion-exchange followed by gel-filtration chromatography separated two purified fractions: LBP-1 (115.1 kDa) and LBP-2 (73.5 kDa). LBP-1 exhibited a compact morphology and featured spectral characteristics indicative of a triple-helix structure, along with both α- and <

SUBMITTER: Chen Y 

PROVIDER: S-EPMC12662143 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature