Metabolomic and microbial diversity perspectives on <i>Aspergillus oryzae</i> culture-induced modifications in ovine feed utilization and rumen ecosystem.

ABSTRACT:

Introduction

Aspergillus oryzae culture (AOC) is widely used as a feed additive to enhance ruminant productivity and rumen function. However, the underlying mechanisms at the microbiome-metabolome interface remain poorly understood. This study aimed to elucidate how dietary AOC supplementation influences sheep production performance, rumen fermentation, microbial communities, and metabolomic profiles.

Methods

Twelve rumen-fistulated sheep were randomly assigned to a control group (basal diet) and a trial group (basal diet + 1% AOC). The experiment lasted 30 days, during which production performance, nutrient digestibility, ruminal pH, volatile fatty acids (VFA), ammonia nitrogen, microbial diversity (16S rDNA sequencing), and metabolomic profiles (LC-MS) were systematically

SUBMITTER: Xie J 

PROVIDER: S-EPMC12662177 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature