Evaluation of immune protection of a multi-antigenic DNA vaccine encoding TgROP6 and TgMIC12 against <i>Toxoplasma gondii</i> infection.

ABSTRACT:

Introduction

Toxoplasma gondii infection causes severe congenital disease and abortion in humans and animals. This study evaluated a novel multivalent DNA vaccine targeting ROP6, and MIC12 for protection against acute (RH strain) and chronic (PRU strain) toxoplasmosis in mouse models.

Methods

Eukaryotic plasmids encoding pVAX-ROP6 and pVAX-MIC12 were constructed, and mice were immunized with either single or combined formulations.

Results and discussion

Vaccination elicited a robust Th1-biased immune response, characterized by elevated IgG2a/IgG1 ratios, enhanced cytotoxic T lymphocyte activity, increased CD4+ and CD8+ T cell populations, and elevated production of IFN-γ, IL-12, and IL-2. The dual vaccine demonstrated superior efficacy, significantly pro

SUBMITTER: Xu B 

PROVIDER: S-EPMC12662228 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature