Single-cell transcriptome analysis elucidates the microenvironmental interactions between colorectal cancer and sarcopenia.

ABSTRACT: Colorectal cancer (CRC) often occurs in combination with sarcopenia (SP), which affects the prognosis of CRC, but the histopathological mechanisms underlying this process are unclear. Single-cell transcriptome technology provides a tool for resolving multi-organ pathological interactions, and previous studies have lacked direct molecular evidence of a trans-tissue microenvironment. To address this, we applied an integrated analytical approach. The integration of single-cell transcriptome data from 16 CRC tumor tissues (scRNA-seq) and 17 SP muscle tissues (snRNA-seq) was used to systematically resolve heterogeneity across tissue microenvironments using Harmony to correct for batch effects and combining cellular annotations, differential expression analyses, and CellChat communication modeli

SUBMITTER: Qiu T 

PROVIDER: S-EPMC12662417 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature