Identification of sus-PSMB7_0001 as a potential pro-angiogenic circular RNA in neonatal pig hearts.

ABSTRACT: While circular RNAs (circRNAs) regulating angiogenesis have been identified in fish and rodent hearts, their expression profiles in pig hearts remain largely unknown. This study aims to identify circRNAs that regulate angiogenesis in postnatal pig hearts. Total RNA sequencing data on pig heart tissues collected on postnatal days 1 (P1), 3 (P3), 7 (P7) and 28 (P28) were previously reported. This study analyzed conserved circRNAs associated with angiogenesis in the database. Functional studies were conducted in human umbilical vein endothelial cells (HUVECs) and hiPSC-derived endothelial cells (hiPSC-ECs). siRNA-mediated knockdown of circRNAs and miRNAs was performed to validate their functions in regulating angiogenesis. Fluorescence in situ hybridization was used to examine circRNA localiz

SUBMITTER: Shi H 

PROVIDER: S-EPMC12662648 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature