A microscopy-based CRISPR screening platform enables organellar functional genomics and illuminates ciliary biology.

ABSTRACT: Microscopy offers an indispensable technique for visualizing biological processes and for defining cytological abnormalities characteristic of disease. However, combining microscopy with the power of pooled CRISPR screening presents considerable technical challenges, hindering application of systematic genetic analysis to imaging-defined phenotypes. Here, we establish a fluorescence microscopy-based CRISPR screening platform that combines ease of implementation with flexible analysis of live-cell or antibody-based molecular markers, including post-translational modifications. Applying this methodology, we systematically identify regulators of primary cilium structure and function in human cells through targeted and genome-wide screens. We further show that integration of screens focused on

SUBMITTER: Sun J 

PROVIDER: S-EPMC12662653 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature