Transcriptomic profile of BpsR4 and its roles in stress response, antibiotic susceptibility, biofilm formation, and pathogenesis in <i>Burkholderia pseudomallei</i>.

ABSTRACT: ObjectiveThe study investigated the role of the quorum sensing (QS) regulator BpsR4 in the physiology and virulence of Burkholderia pseudomallei strain K96243.MethodsA B. pseudomallei bpsR4 knockout mutant (ΔbpsR4) was constructed, and its gene expression profile was compared with that of the wild-type (WT) K96243 strain using RNA sequencing. In vitro assays were conducted to assess growth rates, stress responses, antibiotic sensitivity, motility, and biofilm formation. Ex vivo pathogenicity was evaluated using a human skin fibroblast infection model, and in vivo virulence was assessed in the Galleria mellonella model.ResultsbpsR4 deletion led to significant transcriptional reprogramming, including the downregulation of genes involved

SUBMITTER: Rungruengkitkun A 

PROVIDER: S-EPMC12663075 | biostudies-literature | 2025 Oct-Dec

REPOSITORIES: biostudies-literature