Patient-derived tumoroids recapitulate the morphologic and molecular features of pediatric brain tumors.

ABSTRACT: Pediatric brain tumors (PBTs) are the leading cause of cancer-related mortality in children. Despite advances in next-generation sequencing (NGS) deepening our understanding of the molecular features of PBT, the lack of preclinical models that capture their complexity and diversity remains a significant barrier to developing less toxic and more effective treatments. We have established 20 ex-vivo patient-derived tumoroid (PDTs) cultures from fresh surgical material of a wide range of PBTs, including low- and high-grade gliomas, medulloblastomas, and even rarer tumor entities such as a CNS tumor with BCOR alteration. Immunofluorescence, NGS analysis, and DNA methylation profiling revealed that the PDTs faithfully mirrored the cellular nature, the genetic and epigenetic landscape of their ma

SUBMITTER: Soares-Ferreira B 

PROVIDER: S-EPMC12663098 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature