Assessment of cytotoxicity of some synthetic compounds against breast carcinoma spheroids with subsequent analysis of pro-apoptotic and gene expression.

ABSTRACT: The current study examined the anticancer potential of 80 synthetic organic compounds at 50µM using in vitro two-dimensional (2D) and three-dimensional (3D) spheroids model breast adenocarcinoma (MCF7) models. The most promising six cytotoxic compounds on the 3D spheroids model (> 70% growth cytotoxicity) were counter-screened for their safety on normal cells (RPE1 cell line) grown as 3D spheroids. In parallel, the six compounds were tested for their pro-apoptotic activity using the M30 Cyto death ELISA assay. Based on the activity on MCF7 3D spheroids model, half maximum inhibitory concentration (IC₅₀ < 26µM), the safety on normal cells (< 35% cytotoxicity) and the pro-apoptotic activity on cancer cells (> fourfold increase of negative control), three compounds were considered

SUBMITTER: Mahmoud K 

PROVIDER: S-EPMC12663132 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature