Tracking copper-zinc and manganese superoxide dismutase in Avicennia marina reveals time-dependent expression of SOD isoforms in response to salt and lead stress.

ABSTRACT: Salinity and heavy metals significantly impact plant growth and development. However, mangroves demonstrate remarkable tolerance to these stresses. This study focuses on tracking superoxide dismutase (SOD), a key enzyme responsible for neutralizing superoxide anions. Gene expression analysis revealed that the genes encoding CuZnSOD (AmSOD1) and MnSOD are upregulated in Avicennia marina seedlings exposed to salt and lead stress. To investigate the protein-level expression of these isoforms, CuZnSOD and MnSOD (AmSOD2) from A. marina were heterologously expressed, purified, and used to produce specific antibodies in rabbits. These antibodies successfully distinguished between the two isoforms. Using 2D gel electrophoresis, western blotting combined with mass spectrometry, AmSOD1 was identifie

SUBMITTER: Mahdavian M 

PROVIDER: S-EPMC12663245 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature