Stability analysis of reference genes in Maconellicoccus hirsutus across various conditions including dsRNA treatment.

ABSTRACT: Maconellicoccus hirsutus is a polyphagous pest globally distributed in tropical and subtropical regions. In India, it is reported to adversely affect commercially significant crops, including grapes, guava, custard apple, and mulberry. Gene expression studies have become crucial for comprehending gene function and understanding molecular pathways, which are essential for developing effective pest management strategies. The RT-qPCR is a highly sensitive technique used to study the expression patterns of target genes. However, it's crucial to use a suitable internal reference gene, which is often overlooked before delving into the analysis of gene expression via RT-qPCR. The present study evaluates the expression stability of six candidate reference genes (28S, ATP51a, β-tubulin, GAPDH, α-tu

SUBMITTER: Negi N 

PROVIDER: S-EPMC12663401 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature