A proximity map of RAB GTPases delineates roles for RAB14 in EARP complex and UHRF1BP1 endosomal recruitments.

ABSTRACT: RAB GTPases, the most abundant small GTPase family, regulate membrane trafficking, including cargo sorting, vesicle budding, transport, docking, and fusion. Guanine exchange factors and GTPase-activating proteins control their activity. Activated RABs recruit effectors to mediate trafficking, but identifying RAB-associated proteins is challenging due to the transient nature of their interactions. Recent proximity labeling techniques enable covalent tagging of nearby proteins, allowing for comprehensive mapping of RAB GTPases neighboring proteins. Here we report APEX2 proximity labeling of 23 human RABs and their neighboring proteomes. Bioinformatic analyses revealed proximal proteins for a broad range of RABs. Notably, we identified a novel interaction between RAB25 and DENND6A, with RAB25

SUBMITTER: Gaudreault V 

PROVIDER: S-EPMC12663406 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature