Methanol biotransformation for the production of biodegradable plastic monomer L-lactate in yeast.

ABSTRACT: Methanol is an ideal feedstock for biomanufacturing, and production of the biodegradable plastic monomer lactate from methanol is a promising approach for mitigating white pollution. However, it is challenging to engineer microbes for lactate production from methanol because of strong competition between product synthesis and cell growth. Here, Ogataea polymorpha is rewired to synthesize L-lactate from methanol, where the cofactor ratio of NADPH/NADP⁺ is higher than that of NADH/NAD⁺. By engineering gene expression, enhancing cell viability, modifying cofactor homeostasis, and performing mitochondrial compartmentalization, 2.5 g/L L-lactate is produced in a shake flask. Fed-batch fermentation in a bioreactor results in the highest titer of 25.0 g/L L-lactate from meth

SUBMITTER: Yu W 

PROVIDER: S-EPMC12663439 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature