Protein deglycase DJ-1 deficiency aggravates acute viral myocarditis by promoting apoptosis via reducing Dusp1 expression.

ABSTRACT: Myocardial apoptosis is a cardinal process in acute viral myocarditis (VMC) instigated by coxsackievirus B3 (CVB3) infection. The anti-apoptotic regulator protein deglycase DJ-1 (DJ-1) is implicated in several pathological processes, yet its role in CVB3-induced VMC remains obscure. In this study, we decipher the protective role of DJ-1 against VMC. We found decreased DJ-1 expression in CVB3-infected H9C2 cells and VMC mouse heart tissues. DJ-1 knockout exacerbated VMC severity and apoptosis. Conversely, recovery of DJ-1 levels showed protective effects in vitro and in vivo. We observed downregulation of Dusp1 in DJ-1-deficient mice hearts and H9C2 cells with DJ-1 silencing. Subsequent in vitro assays corroborated that downregulation of DJ-1 amplifies apoptosis in H9C2 cells following CVB3

SUBMITTER: Yan P 

PROVIDER: S-EPMC12663478 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature