Improvement mechanism of cellulose nanocrystal in coordination with calcium ions on the thermal gelation of soybean protein amyloid fibrils.

ABSTRACT: This study investigated the mechanism by which cellulose nanocrystals (CNC) derived from pear peel pomace synergize with Ca²⁺ to regulate the thermal gelation of soy protein amyloid fibrils (SAFs). Results showed that the combined use of 0.09 % (w/v) CNC and 400 mmol Ca²⁺ significantly enhanced SAFs gel strength and viscoelasticity, promoting the formation of β-sheet conformation and exposure of aromatic amino acid residues in SAFs. CNC reinforced hydrogen bonds to form excellent water-holding gel units, whereas Ca²⁺ strengthened ionic bonds and hydrophobic interactions to construct low water-holding gels with high strength. Mechanistic analysis revealed that CNC binds to specific SAFs amino acid sites via van der Waals forces and hydrogen bon

SUBMITTER: Zhu S 

PROVIDER: S-EPMC12663481 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature