Comparison of LC-MS methods for the quantitation of ciguatoxins in fish - A collaborative study.

ABSTRACT: Ciguatoxins (CTXs) are marine biotoxins that can contaminate seafood and if consumed can result in ciguatera poisoning (CP). The analysis of CTXs is challenging, as they occur in complex tissue matrices, cause CP symptoms at trace amounts (<1 μg kg^-1), and certified reference materials are not available. Currently, no standard operating protocols exist for sample preparation or instrumental analysis. Five laboratories worldwide participated in this first-time method comparison study, which used sample extracts containing different CTX groups (CTX4A group, CTX3C group, C-CTX-1) to identify factors impacting CTX quantitation using LC-MS/MS and LC-HRMS. Matrix effects were found to significantly influence CTX quantitation, along with factors such as instrument, eluents, or selected

SUBMITTER: Spielmeyer A 

PROVIDER: S-EPMC12663502 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature