FBXO5 alleviates apical periodontitis by facilitating TP53 protein degradation.

ABSTRACT:

Background

Apical periodontitis (AP) is a common inflammatory disease that irreversibly damages apical papilla tissue and hinders dental regeneration. The molecular mechanisms underlying F-box protein 5 (FBXO5)- and tumor protein p53 (TP53)-mediated regulation of osteogenic differentiation in human stem cells from the apical papilla (hSCAPs) remain to be fully elucidated, particularly in the context of AP pathogenesis.

Methods

Differentially expressed genes in the AP tissues from AP patients and normal controls were identified through bioinformatics analysis. The hSCAPs were isolated from the third molars of healthy human individuals. Alkaline phosphatase (ALP) staining and ALP activity assay, along with Alizarin Red S (ARS) staining, were performed to assess osteogenic diff

SUBMITTER: Zhang R 

PROVIDER: S-EPMC12663516 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature