An apical ring protein essential for conoid complex assembly and daughter cell formation in Toxoplasma gondii.

ABSTRACT: In Toxoplasma gondii, the conoid complex consists of intraconoidal microtubules (ICMTs), preconoidal rings (PCRs), apical polar ring (APR), and the conoid. This organelle plays an important role for initiation of gliding motility, required for host cell invasion and egress. The molecular mechanisms governing stepwise assembly of the conoid complex remain poorly understood. We previously identified CGP, an essential protein required for motility initiation. Here, we demonstrate that CGP is crucial for anchoring FRM1 and other PCR components to mature PCRs, while the initial assembly in daughter cells is unaffected. Cryo-electron tomography of CGP-depleted parasites reveals the absence of the PCRs in the mature parasites, demonstrating that CGP is essential for stabilising the PCRs after rep

SUBMITTER: Li W 

PROVIDER: S-EPMC12663574 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature