MYC-mediated transcriptional control of splicing factors promotes RNA splicing in activated B lymphocytes.

ABSTRACT: Germinal center B cells play a pivotal role in the generation of long-lived plasma cells and high-affinity antibodies. Although RNA splicing and alternative splicing are known to regulate germinal center B cell responses, the upstream mechanisms controlling these splicing events remain poorly understood. RNA sequencing analysis revealed significant upregulation of alternative splicing factors and core spliceosome genes in germinal center B cells. Genome-wide binding analyses using CUT&RUN and ChIP-seq confirmed that MAX and MYC physically bind to the promoters of these splicing regulators. High MYC expression in B cells markedly upregulated multiple splicing factors, whereas MAX knockout substantially suppressed their expression. Through splicing event profiling, we demonstrated that MYC p

SUBMITTER: Li S 

PROVIDER: S-EPMC12663575 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature