Inhibition of Histone Methyltransferase G9a Activates Autophagy and Provides Protection in Dry Eye Disease.

ABSTRACT:

Purpose

This study aimed to investigate the expression dynamics and functional role of the histone methyltransferase G9a in dry eye disease (DED).

Methods

An in vitro DED model was established using human corneal epithelial cells (HCECs) cultured in hyperosmotic medium (90-mM NaCl). For the in vivo model, mice were treated with 0.075% benzalkonium chloride eye drops twice daily for 14 days. Phenotypic and mechanistic analyses were conducted using quantitative real-time PCR, western blotting, immunofluorescence staining, flow cytometry, and chromatin immunoprecipitation assays. Disease severity was assessed by fluorescein sodium staining and the phenol red thread test. Histopathological analysis included periodic acid-Schiff staining, hematoxylin and eosin staining, and immun

SUBMITTER: Chen Z 

PROVIDER: S-EPMC12663890 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature