Intracameral Puncture Lowers Intraocular Pressure and Triggers an Immune Response in the Conventional Outflow Tract.

ABSTRACT:

Purpose

Intracameral injection is an effective delivery method for biomedical agents and therapeutics to conventional outflow tract tissues. However, the effect of intracameral injections on intraocular pressure (IOP) and aqueous dynamics has not been well characterized, warranting further investigation.

Methods

Wild type 3 to 5-month-old C57BL/6 mice were subjected to intracameral puncture (ICP; without injection of any material). Following ICP, IOP, outflow facility, aqueous production, episcleral vessel diameter, and macrophage densities were measured.

Results

On day 1, IOP was significantly reduced by 30% (P < 0.0001, n = 25), whereas outflow facility (P = 0.31, n = 15) and aqueous production (P = 0.16, n = 9) were unchanged. As well, Schlemm's canal filtration ar

SUBMITTER: Schuman DM 

PROVIDER: S-EPMC12663893 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature