Detection of PIP₂ distributions in biological membranes using a peptide-based sensor.

ABSTRACT: Organization and composition of the plasma membrane are important modulators of many cellular programs. Phosphatidylinositol phosphate (PIP) lipids are low-abundance membrane constituents with different arrangements of phosphate groups around an inositol head group, regulating many signaling pathways. Numerous strategies have been developed to detect and track PIP species to monitor their clustering, mobility, and interactions with binding partners. We implement a peptide-based, ratiometric sensor for the detection of PI(4,5)P₂ lipids in reconstituted membrane systems that permit absolute quantification of PI(4,5)P₂ densities down to physiological levels less than 4 mol per cent. The sensor is membrane-permeable and easily transferable to measurements in living cells.

SUBMITTER: Menon VK 

PROVIDER: S-EPMC12664033 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature