Primary cilium and TULP3-dependent ciliary targeting of ACE2 in SARS-CoV-2 tropism.

ABSTRACT:

Background

Viruses initiate infection by engaging specific receptors on the host cell surface. While the surface receptor ACE2 mediates SARS-CoV-2 entry, the precise role of subcellular trafficking, particularly, the exact involvement of primary cilium trafficking in viral entry remains largely unresolved.

Methods

We used in-vitro human cell models and SARS-CoV-2 pseudoviruses to elucidate the viral attachment and host cell entry mechanisms. Mechanistic studies were conducted using a multidimensional approach that combined flow cytometry analysis, co-immunofluorescence and confocal microscopy, co-immunoprecipitation, genetic manipulations and ciliary perturbations, and structural predictions.

Results

Our study uncovers Tubby Like Protein-3 (TULP3) as a pivotal ciliary

SUBMITTER: Du YH 

PROVIDER: S-EPMC12664140 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature