Computational development of multi-epitope vaccine to induce adaptive immunity against multi-drug resistant Prevotella intermedia.

ABSTRACT: Prevotella intermedia is a gram-negative, anaerobic, multidrug-resistant bacterium known to cause oral infections. Given its clinical significance as an opportunistic pathogen, developing effective preventive and therapeutic measures is crucial. This study employed an integrated approach, combining subtractive proteomics and immunoinformatics, to identify novel therapeutic targets. We analyzed the core genome of P. intermedia, revealing five essential proteins as potential drug targets and three as potential candidates for a vaccine. The selected vaccine candidate proteins underwent additional evaluation to determine their immunogenic epitopes (B-cell, MHC-I, and MHC-II) and the design multi-epitope vaccine incorporating suitable linkers and adjuvants. Structural validation, including the

SUBMITTER: Kanwal A 

PROVIDER: S-EPMC12664192 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature