Assembly and comparative analysis of chromosomal mitochondrial genomes in multiple Medicago species.

ABSTRACT:

Background

Medicago is an economically important forage genus widely distributed across China, yet its mitochondrial genomes remain poorly understood. Comprehensive characterization of mitochondrial genome structure, function, and evolution is crucial for elucidating plant biological mechanisms, improving germplasm utilization, and advancing molecular breeding.

Results

We assembled and compared the mitochondrial genomes of eight Medicago species, including six newly sequenced genomes. Five species contained typical single circular mitochondrial genomes, whereas M. falcata, M. platycarpos, and M. sativa displayed complex multipartite circular conformations. Genome sizes ranged from 281,240 to 356,577 bp, with 55-74 functional genes. Repeat analysis identified 141 simple seque

SUBMITTER: Yang R 

PROVIDER: S-EPMC12664221 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature