Microarray analyses of gene expression during chondrocyte differentiation identifies novel regulators of hypertrophy.

ABSTRACT: Ordered chondrocyte differentiation and maturation is required for normal skeletal development, but the intracellular pathways regulating this process remain largely unclear. We used Affymetrix microarrays to examine temporal gene expression patterns during chondrogenic differentiation in a mouse micromass culture system. Robust normalization of the data identified 3300 differentially expressed probe sets, which corresponds to 1772, 481, and 249 probe sets exhibiting minimum 2-, 5-, and 10-fold changes over the time period, respectively. GeneOntology annotations for molecular function show changes in the expression of molecules involved in transcriptional regulation and signal transduction among others. The expression of identified markers was confirmed by RT-PCR, and cluster analysis reve

SUBMITTER: James CG 

PROVIDER: S-EPMC1266429 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature