Global genome analysis identifies glycolipids and lipoteichoic acid alanylation as contributors to group B streptococcal diabetic wound infection.

ABSTRACT: Diabetic individuals frequently develop chronic, hyper-inflammatory, non-healing wounds, which are the leading cause of all non-traumatic amputations. Group B Streptococcus (GBS) is a prominent bacterium isolated from diabetic wound infections, and in a murine model of diabetic wound infection, GBS stimulates an influx of neutrophils into the wound. Utilizing a transposon mutant screen, we identified 291 bacterial genes required for fitness during diabetic wound infection, including enzymes involved in glycolipid biosynthesis and lipoteichoic acid (LTA) alanylation. GBS mutants lacking either LTA alanylation (ΔdltA) or all glycolipids (ΔiagB) are attenuated in murine diabetic wound infection. GBS induces primary and secondary degranulation in primary human neutrophils, and the ΔiagB mutant

SUBMITTER: Joyce LR 

PROVIDER: S-EPMC12664321 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature