Transcriptome and chromatin accessibility divergence during differentiation of a bipotential progenitor cell population to erythroblasts and megakaryocytes.

ABSTRACT: Changes in gene expression drive differentiation along cell lineages, and shifts in gene expression are associated with alterations in chromatin accessibility reflecting activation or repression. We used deep sequencing of polyadenylated RNA to map the transcriptomes of the megakaryocyte-erythroid progenitor (MEP) and its two daughter lineages, erythroblasts (ERYs) and megakaryocytes (MEGs), in mice to reveal insights into differentiation. Transcriptome comparisons revealed that MEPs already expressed much of the MEG program while continuing to express genes associated with parallel myeloid lineages. By contrast, ERYs underwent an extensive program of gene induction along with repression of pan-hematopoietic and MEG genes. Maps of transcription factor occupancy also indicated distinct mode

SUBMITTER: Mishra T 

PROVIDER: S-EPMC12664380 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature