A transmission electron microscopy platform for assessing mitochondrial and nuclear architecture in cardiomyocytes.

ABSTRACT: Mitochondria are central to cardiomyocyte function, and their spatial organization regulates nuclear signaling and gene transcription, holding potential for novel cardioprotective interventions. We developed a transmission electron microscopy platform optimized for resolving mitochondrial subpopulations and nuclear architecture in adult cardiomyocytes. This approach reliably captures longitudinal sections containing the center of the nucleus and perinuclear regions, enabling consistent imaging of subcellular nanostructures, assessment of pharmacological effects within the same organism, and visualization of extracellular vesicles carrying dysfunctional mitochondria. Integrated with an analysis workflow employing machine learning-based segmentation for annotation, the method allows automate

SUBMITTER: Kiessling M 

PROVIDER: S-EPMC12664883 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature