Transcription Factor GmERFA Interacts With GmNFYA and Acts as a Negative Regulator of Seed Fatty Acid Accumulation in Soybean.

ABSTRACT: Soybean is one of the most important oilseed crops, and its seed oil content directly determines the economic value and industrial applicability worldwide. However, how soybean seed oil accumulation is regulated remains less understood. Here, through RNA-seq analysis and screening for the interacting proteins of a positive oil regulator GmNFYA, we identified an AP2/ERF-type transcription factor GmERFA, which acts as a negative regulator of oil accumulation. Knocking out GmERFA and its homologue by genome editing increased seed total fatty acid content, while overexpression of GmERFA leads to a reduced fatty acid level in transgenic soybean. GmERFA interacts with GmNFYA to inhibit its transcriptional activation of GmbZIP123 and GmZF392, both of which promotes seed oil accumulation. The GmER

SUBMITTER: Liu Y 

PROVIDER: S-EPMC12665053 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature