Phase Separation of PHLDB2 Drives EMT and Tumor Progression in Triple-Negative Breast Cancer.

ABSTRACT:

Background

Liquid-liquid phase separation (LLPS) has emerged as a critical mechanism underlying oncogenic signaling and transcriptional regulation; however, its involvement in triple-negative breast cancer (TNBC) remains largely unknown. The pleckstrin homology-like domain family B member 2 (PHLDB2) has been implicated in cytoskeletal organization and cell migration, but its role in phase separation-mediated tumor progression has not been explored.

Methods

We conducted an integrative multi-omics analysis combining transcriptomic profiling, bioinformatic modeling, and molecular biology experiments to elucidate the function of PHLDB2 in TNBC. Expression, mutation, and copy number variation of phase separation-related genes were analyzed using TCGA, METABRIC, and GTEx datasets.

SUBMITTER: Zhang M 

PROVIDER: S-EPMC12665185 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature