Application analysis of two nucleic acid detection systems in blood detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus.

ABSTRACT:

Objective

This study aimed to compare the diagnostic efficacy between the Kehua and Roche nucleic acid testing (NAT) systems for detecting hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus (HIV) in blood donation screening.

Methods

We analyzed retrospective data from 670,775 transient blood donation samples collected between 2016 and 2024. Key performance indicators (KPIs) included the types of reactive samples, effective split rate, effective reaction rate, single pool invalid number, batch invalid number, and pooling missing samples. Annual trends in cycle threshold (CT) value distributions and overall system performance were also evaluated.

Results

Among 419 reactive samples (Kehua: 202; Roche: 217), no significant differences were

SUBMITTER: Wang T 

PROVIDER: S-EPMC12665262 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature