Hydrogen regulates the aryl hydrocarbon receptor, improving bronchopulmonary dysplasia in neonatal rats and RLE-6TN cells exposed to hyperoxia.

ABSTRACT:

Introduction

This study investigates the role and underlying mechanism of hydrogen (H₂) in hyperoxia-induced bronchopulmonary dysplasia (BPD), aiming to provide a theoretical foundation for developing effective BPD treatment strategies.

Methods

A hyperoxia-induced BPD rat model and a rat type II alveolar epithelial cell (RLE-6TN) injury model were established. H₂ was administered to assess its effects on BPD rats, while hydrogen-rich medium was used to treat RLE-6TN cells to evaluate cell viability. In vivo and in vitro experiments were conducted to explore the regulatory influence of H₂ on the aryl hydrocarbon receptor (AHR). Additionally, AHR knockdown and overexpression experiments were performed to determine the impact of AHR on cell viability.

Results</

SUBMITTER: Liang M 

PROVIDER: S-EPMC12665534 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature