Effect of bovine oocyte transportation system on embryonic quality.

ABSTRACT: The conventional ovum pick-up method requires oocytes to be transported from local farms to the laboratory, where they undergo nuclear maturation. However, atmospheric conditions for oocyte transportation differ from those for normal oocyte maturation in vitro. In this study, we examined the effects of conventional and modified oocyte transport conditions on oocyte quality and subsequent embryonic development. Cumulus-oocyte complexes were collected from slaughterhouse-derived bovine ovaries and cultured in few drops of medium on plastic plates in a CO₂-incubator (Control), in plastic tubes containing medium (C-T) in air, or in tubes containing gellan gum and medium (MC-T) in air. C-T conditions reduced mitochondrial functionality (mitochondrial membrane potential and adenosine

SUBMITTER: Hara S 

PROVIDER: S-EPMC12665968 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature