Characterisation of four novel bacteriophages targeting carbapenem-resistant <i>Klebsiella pneumoniae</i> and their lytic activity alone and in combination.

ABSTRACT: Klebsiella pneumoniae is a nosocomial pathogen with rising levels of antibiotic resistance, increasing interest in bacteriophage therapy as an adjunct or alternative treatment. This study aimed to isolate and characterise bacteriophages active against multidrug-resistant K. pneumoniae and evaluate their efficacy. Four lytic phages, Kilian, Trimon, Jurek, and Olmo were isolated from water sources using K. pneumoniae ATCC BAA-2146 as host. Genome analysis revealed no known lysogeny, antibiotic resistance, or virulence-associated genes, supporting their suitability for therapeutic use. Electron microscopy classified Kilian, Trimon, and Jurek as siphoviruses, and Olmo as a myovirus. All phages showed rapid adsorption (2.5-5 min), short latent periods (5-15 min), and variab

SUBMITTER: Fausti E 

PROVIDER: S-EPMC12666361 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature