Single-cell transcriptome analysis profiles cellular dynamics and transcriptional changes in diabetic wound tissues following ESWT treatment.

ABSTRACT:

Introduction

Diabetic wounds (DWs) remain a major complication of diabetes mellitus, characterized by impaired healing and limited therapeutic options. Extracorporeal shock wave therapy (ESWT), a non-invasive physical modality, has recently shown promise in accelerating chronic wound repair, yet the underlying cellular mechanisms remain poorly understood.

Methods

Here, we employed single-cell RNA sequencing (scRNA-seq) to construct a comprehensive cellular atlas of DW tissues treated with ESWT, profiling approximately 39,475 cells.

Results

Our analysis identified 12 major cell populations, including macrophages, fibroblasts, endothelial cells, keratinocytes, and immune subsets, and revealed widespread transcriptional reprogramming associated with ESWT treatment. ESWT

SUBMITTER: Li D 

PROVIDER: S-EPMC12666372 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature