Populus VariantDB v3.2 facilitates CRISPR and functional genomics research.

ABSTRACT: The success of CRISPR genome editing studies depends critically on the precision of guide RNA (gRNA) design. Sequence polymorphisms in outcrossing tree species pose design hazards that can render CRISPR genome editing ineffective. Despite recent advances in tree genome sequencing with haplotype resolution, sequence polymorphism information remains largely inaccessible to various functional genomics research efforts. The Populus VariantDB v3.2 addresses these challenges by providing a user-friendly search engine to query sequence polymorphisms of heterozygous genomes. The database accepts short sequences, such as gRNAs and primers, as input for searching against multiple poplar genomes, including hybrids, with customizable parameters. We provide examples to showcase the utilities of Variant

SUBMITTER: Zhou R 

PROVIDER: S-EPMC12666374 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature